High-Throughput Sequencing of Oral Microbiota in Candida Carriage Sjogren's Syndrome Patients: A Pilot Cross-Sectional Study.

ABSTRACT:

Background

This study sought to characterize the saliva microbiota of Candida carriage Sjögren's syndrome (SS) patients compared to oral candidiasis and healthy patients by high-throughput sequencing.

Methods

Fifteen patients were included, with five Candida carriage SS patients (decayed, missing, and filled teeth (DMFT) score 22), five oral candidiasis patients (DMFT score 17), and five caries active healthy patients (DMFT score 14). Bacterial 16S rRNA was extracted from rinsed whole saliva. PCR amplification generated DNA amplicons of the V3-V4 hypervariable region, which were sequenced on an Illumina HiSeq 2500 sequencing platform and compared and aligned to the SILVA database. Taxonomy abundance and community structure diversity was analyzed using Mothur so

SUBMITTER: Xing H 

PROVIDER: S-EPMC9964208 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature