Landscape of Well-Coordinated Fracture Healing in a Mouse Model Using Molecular and Cellular Analysis.

ABSTRACT: The success of fracture healing relies on overlapping but coordinated cellular and molecular events. Characterizing an outline of differential gene regulation throughout successful healing is essential for identifying crucial phase-specific markers and may serve as the basis for engineering these in challenging healing situations. This study analyzed the healing progression of a standard closed femoral fracture model in C57BL/6N (age = 8 weeks) wild-type male mice. The fracture callus was assessed across various days post fracture (D = days 0, 3, 7, 10, 14, 21, and 28) by microarray, with D0 serving as a control. Histological analyses were carried out on samples from D7 until D28 to support the molecular findings. Microarray analysis revealed a differential regulation of immune response, a

SUBMITTER: Malhan D 

PROVIDER: S-EPMC9964763 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature