Marine-Derived Natural Product HDYL-GQQ-495 Targets P62 to Inhibit Autophagy.

ABSTRACT: Autophagy is widely implicated in pathophysiological processes such as tumors and metabolic and neurodegenerative disorders, making it an attractive target for drug discovery. Several chemical screening approaches have been developed to uncover autophagy-modulating compounds. However, the modulation capacity of marine compounds with significant pharmacological activities is largely unknown. We constructed an EGFP^KI-LC3B cell line using the CRISPR/Cas9 knock-in strategy in which green fluorescence indicated endogenous autophagy regulation. Using this cell line, we screened a compound library of approximately 500 marine natural products and analogues to investigate molecules that altered the EGFP fluorescence. We identified eight potential candidates that enhanced EGFP fluorescenc

SUBMITTER: Li Q 

PROVIDER: S-EPMC9965018 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature