Tailoring the Host Range of Ackermannviridae Bacteriophages through Chimeric Tailspike Proteins.

ABSTRACT: Host range is a major determinant in the industrial utility of a bacteriophage. A model host range permits broad recognition across serovars of a target bacterium while avoiding cross-reactivity with commensal microbiota. Searching for a naturally occurring bacteriophage with ideal host ranges is challenging, time-consuming, and restrictive. To address this, SPTD1.NL, a previously published luciferase reporter bacteriophage for Salmonella, was used to investigate manipulation of host range through receptor-binding protein engineering. Similar to related members of the Ackermannviridae bacteriophage family, SPTD1.NL possessed a receptor-binding protein gene cluster encoding four tailspike proteins, TSP1-4. Investigation of the native gene cluster through chimeric proteins iden

SUBMITTER: Gil J 

PROVIDER: S-EPMC9965104 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature