Enhancement of Pathogen Toxicity by Feeding Reticulitermes chinensis Snyder Sonicated Bacteria Expressing Double-Stranded RNA That Interferes with Olfaction.

ABSTRACT: Reticulitermes chinensis Snyder is a serious pest in China, and the odorant receptor co-receptor gene RcOrco plays a crucial role in olfaction. However, the function of RcOrco in the resistance of termites to entomopathogens has not been reported. We constructed dsRcOrco-HT115 engineered bacteria based on the RcOrco sequence from the full-length transcriptome data of R. chinensis. The engineered bacteria expressed dsRNA of RcOrco. Sonication was used to inactivate the dsRNA-HT115 strain and obtain a large amount of dsRcOrco. The dsRcOrco produced using this method overcame the problem that genetically engineered bacteria could not be applied directly and improved its effectiveness against termites. Bioassays using the dsRcOr

SUBMITTER: Jiang D 

PROVIDER: S-EPMC9965219 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature