Movement of Chronic Wasting Disease Prions in Prairie, Boreal and Alpine Soils.

ABSTRACT: Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy negatively impacting cervids on three continents. Soil can serve as a reservoir for horizontal transmission of CWD by interaction with the infectious prion protein (PrP^CWD) shed by diseased individuals and from infected carcasses. We investigated the pathways for PrP^CWD migration in soil profiles using lab-scale soil columns, comparing PrP^CWD migration through pure soil minerals (quartz, illite and montmorillonite), and diverse soils from boreal (Luvisol, Brunisol) and prairie (Chernozem) regions. We analyzed the leachate of the soil columns by immunoblot and protein misfolding cyclic amplification (PMCA) and detected PrP in the leachates of columns composed of quartz, illite, Luviso

SUBMITTER: Kuznetsova A 

PROVIDER: S-EPMC9965917 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature