Detection of Breast Cancer-Specific Extracellular Vesicles with Fiber-Optic SPR Biosensor.

ABSTRACT: Extracellular vesicles (EVs) have attracted great attention as potential biomarkers for cancer diagnostics. Although several technologies have been developed for EV detection, many of them are still not applicable to clinical settings as they rely on complex EV isolation processes, while lacking sensitivity, specificity or standardization. To solve this problem, we have developed a sensitive breast cancer-specific EV detection bioassay directly in blood plasma using a fiber-optic surface plasmon resonance (FO-SPR) biosensor, previously calibrated with recombinant EVs. First, we established a sandwich bioassay to detect SK-BR-3 EVs by functionalizing the FO-SPR probes with anti-HER2 antibodies. A calibration curve was built using an anti-HER2/^Banti-CD9 combination, resulting in a

SUBMITTER: Yildizhan Y 

PROVIDER: S-EPMC9966403 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature