Permeability Assessment of a High-Throughput Mucosal Platform.

ABSTRACT: Permeability across cellular membranes is a key factor that influences absorption and distribution. Before absorption, many drugs must pass through the mucus barrier that covers all the wet surfaces of the human body. Cell-free in vitro tools currently used to evaluate permeability fail to effectively model the complexity of mucosal barriers. Here, we present an in vitro mucosal platform as a possible strategy for assessing permeability in a high-throughput setup. The PermeaPad 96-well plate was used as a permeability system and further coupled to a pathological, tridimensional mucus model. The physicochemical determinants predicting passive diffusion were determined by combining experimental and computational approaches. Drug solubility, size, and shape were found to be the critical prope

SUBMITTER: Butnarasu C 

PROVIDER: S-EPMC9966667 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature