Implementation of Novel Affinity Ligand for Lentiviral Vector Purification.

ABSTRACT: The use of viral vectors as therapeutic products for multiple applications such as vaccines, cancer treatment, or gene therapies, has been growing exponentially. Therefore, improved manufacturing processes are needed to cope with the high number of functional particles required for clinical trials and, eventually, commercialization. Affinity chromatography (AC) can be used to simplify purification processes and generate clinical-grade products with high titer and purity. However, one of the major challenges in the purification of Lentiviral vectors (LVs) using AC is to combine a highly specific ligand with a gentle elution condition assuring the preservation of vector biological activity. In this work, we report for the first time the implementation of an AC resin to specifically purify VS

SUBMITTER: Moreira AS 

PROVIDER: S-EPMC9966744 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature