High-Quality 3D Visualization System for Light-Field Microscopy with Fine-Scale Shape Measurement through Accurate 3D Surface Data.

ABSTRACT: We propose a light-field microscopy display system that provides improved image quality and realistic three-dimensional (3D) measurement information. Our approach acquires both high-resolution two-dimensional (2D) and light-field images of the specimen sequentially. We put forward a matting Laplacian-based depth estimation algorithm to obtain nearly realistic 3D surface data, allowing the calculation of depth data, which is relatively close to the actual surface, and measurement information from the light-field images of specimens. High-reliability area data of the focus measure map and spatial affinity information of the matting Laplacian are used to estimate nearly realistic depths. This process represents a reference value for the light-field microscopy depth range that was not previous

SUBMITTER: Kwon KH 

PROVIDER: S-EPMC9967073 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature