A Four-Step Platform to Optimize Growth Conditions for High-Yield Production of Siderophores in Cyanobacteria.

ABSTRACT: In response to Iron deprivation and in specific environmental conditions, the cyanobacteria Anabaena flos aquae produce siderophores, iron-chelating molecules that in virtue of their interesting environmental and clinical applications, are recently gaining the interest of the pharmaceutical industry. Yields of siderophore recovery from in vitro producing cyanobacterial cultures are, unfortunately, very low and reach most of the times only analytical quantities. We here propose a four-step experimental pipeline for a rapid and inexpensive identification and optimization of growth parameters influencing, at the transcriptional level, siderophore production in Anabaena flos aquae. The four-steps pipeline consists of: (1) identification of the promoter region of the operon of int

SUBMITTER: Kundu K 

PROVIDER: S-EPMC9967094 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature