Safe and Effective Delivery of mRNA Using Modified PEI-Based Lipopolymers.

ABSTRACT: Chemically modified mRNA (modRNA) has proven to be a versatile tool for the treatment of various cancers and infectious diseases due to recent technological advancements. However, a safe and effective delivery system to overcome the complex extracellular and intracellular barriers is required in order to achieve higher therapeutic efficacy and broaden clinical applications. Here, we explored All-Fect and Leu-Fect C as novel transfection reagents derived from lipopolymers, which demonstrated excellent biocompatibility, efficient delivery capabilities, and a robust ability to escape the lysosomes. These properties directly increase mRNA stability by preventing mRNA degradation by nucleases and simultaneously promote efficient gene translation in vitro and in vivo. The modRNA delivered with l

SUBMITTER: Wang H 

PROVIDER: S-EPMC9967631 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature