BdLT-Seq as a barcode decay-based method to unravel lineage-linked transcriptome plasticity.

ABSTRACT: Cell plasticity is a core biological process underlying a myriad of molecular and cellular events taking place throughout organismal development and evolution. It has been postulated that cellular systems thrive to balance the organization of meta-stable states underlying this phenomenon, thereby maintaining a degree of populational homeostasis compatible with an ever-changing environment and, thus, life. Notably, albeit circumstantial evidence has been gathered in favour of the latter conceptual framework, a direct observation of meta-state dynamics and the biological consequences of such a process in generating non-genetic clonal diversity and divergent phenotypic output remains largely unexplored. To fill this void, here we develop a lineage-tracing technology termed Barcode decay Linea

SUBMITTER: Shlyakhtina Y 

PROVIDER: S-EPMC9968323 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature