The translating bacterial ribosome at 1.55 A resolution generated by cryo-EM imaging services.

ABSTRACT: Our understanding of protein synthesis has been conceptualised around the structure and function of the bacterial ribosome. This complex macromolecular machine is the target of important antimicrobial drugs, an integral line of defence against infectious diseases. Here, we describe how open access to cryo-electron microscopy facilities combined with bespoke user support enabled structural determination of the translating ribosome from Escherichia coli at 1.55 Å resolution. The obtained structures allow for direct determination of the rRNA sequence to identify ribosome polymorphism sites in the E. coli strain used in this study and enable interpretation of the ribosomal active and peripheral sites at unprecedented resolution. This includes scarcely populated chimeric hybrid states of the ri

SUBMITTER: Fromm SA 

PROVIDER: S-EPMC9968351 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature