Assessment of distant-site rescue elements for CRISPR toxin-antidote gene drives.

ABSTRACT: Gene drive is a genetic engineering technology that can enable super-mendelian inheritance of specific alleles, allowing them to spread through a population. New gene drive types have increased flexibility, offering options for confined modification or suppression of target populations. Among the most promising are CRISPR toxin-antidote gene drives, which disrupt essential wild-type genes by targeting them with Cas9/gRNA. This results in their removal, increasing the frequency of the drive. All these drives rely on having an effective rescue element, which consists of a recoded version of the target gene. This rescue element can be at the same site as the target gene, maximizing the chance of efficient rescue, or at a distant site, which allows useful options such as easily disrupting anot

SUBMITTER: Chen J 

PROVIDER: S-EPMC9968759 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature