Metabolic engineering of low-pH-tolerant non-model yeast, Issatchenkia orientalis, for production of citramalate.

ABSTRACT: Methyl methacrylate (MMA) is an important petrochemical with many applications. However, its manufacture has a large environmental footprint. Combined biological and chemical synthesis (semisynthesis) may be a promising alternative to reduce both cost and environmental impact, but strains that can produce the MMA precursor (citramalate) at low pH are required. A non-conventional yeast, Issatchenkia orientalis, may prove ideal, as it can survive extremely low pH. Here, we demonstrate the engineering of I. orientalis for citramalate production. Using sequence similarity network analysis and subsequent DNA synthesis, we selected a more active citramalate synthase gene (cimA) variant for expression in I. orientalis. We then adapted a piggyBac transposon system for <

SUBMITTER: Wu ZY 

PROVIDER: S-EPMC9969067 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature