Observation of the protein expression level via naked eye: Pt clusters catalyze non-color molecules into brown-colored molecules in cells.

ABSTRACT: α _v β ₃ is overexpressed in various tumor cells and plays a key role in tumor genesis, invasion, and metastasis. Therefore, it is of great significance to precisely detect the α _v β ₃ level in cells via a simple method. For this purpose, we have constructed a peptide-coated platinum (Pt) cluster. Due to its bright fluorescence, well-defined Pt atom numbers, and peroxidase-like catalytic activity, this cluster can be used to evaluate α _v β ₃ levels in cells by fluorescence imaging, inductively coupled plasma mass spectrometry (ICP-MS), and catalytic amplification of visual dyes, respectively. In this report, the expression level of α _v β ₃ in living cells is well-detected by the naked eye under an ordinary light microscope when the Pt cluster binds to αvβ3 in cells and catalyzes non-color 3,3'-diaminobenzidine (DAB) into brown-colored molecules in situ. Moreover, SiHa, HeLa, and 16HBE cell lines with different α _v β ₃ expression levels can be visually distinguished by the peroxidase-like Pt clusters. This research will provide a reliable method for the simple detection of α _v β ₃ levels in cells.

SUBMITTER: Xia D 

PROVIDER: S-EPMC9969140 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature