Whole-genome sequence analysis of clinically isolated carbapenem resistant Escherichia coli from Iran.

ABSTRACT:

Background

The emergence of carbapenem-resistant Enterobacterales (CRE) continues to threaten public health due to limited therapeutic options. In the current study the incidence of carbapenem resistance among the 104 clinical isolates of Escherichia coli and the genomic features of carbapenem resistant isolates were investigated.

Methods

The susceptibility to imipenem, tigecycline and colistin was tested by broth dilution method. Susceptibility to other classes of antimicrobials was examined by disk diffusion test. The presence of bla_OXA-48, bla_KPC, bla_NDM, and bla_VIM carbapenemase genes was examined by PCR. Molecular characteristics of carbapenem resistant isolates were further investigated by whole-genome sequencing (WGS) using

SUBMITTER: Haeili M 

PROVIDER: S-EPMC9969672 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature