Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle.

ABSTRACT: Many cell biological and biochemical mechanisms controlling the fundamental process of eukaryotic cell division have been identified; however, the temporal dynamics of biosynthetic processes during the cell division cycle are still elusive. Here, we show that key biosynthetic processes are temporally segregated along the cell cycle. Using budding yeast as a model and single-cell methods to dynamically measure metabolic activity, we observe two peaks in protein synthesis, in the G1 and S/G2/M phase, whereas lipid and polysaccharide synthesis peaks only once, during the S/G2/M phase. Integrating the inferred biosynthetic rates into a thermodynamic-stoichiometric metabolic model, we find that this temporal segregation in biosynthetic processes causes flux changes in primary metabolism, with a

SUBMITTER: Takhaveev V 

PROVIDER: S-EPMC9970877 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature