Development of a reverse-transcription droplet digital PCR method for quantitative detection of Cucumber green mottle mosaic virus.

ABSTRACT: Cucumber green mottle mosaic virus (CGMMV) is a re-emerging threat to the production of greenhouse cucumber and other Cucurbitaceae crops worldwide. This seed-borne virus can easily spread from a contaminated seed to seedlings and adjacent plants by mechanical contact between the foliage of diseased and healthy plants, causing extensive yield losses. An accurate method for detecting and quantifying this virus is urgently needed to ensure the safety of the global seed trade. Here, we report the development of a reverse-transcription droplet digital polymerase chain reaction (RT-ddPCR)-based method for specific and high-sensitive detection of CGMMV. By testing three primer-probe sets and optimizing reaction conditions, we showed that the newly developed RT-ddPCR method is highly speci

SUBMITTER: Tian Y 

PROVIDER: S-EPMC9970901 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature