Accelerated germination of aged recalcitrant seeds by K⁺-rich bulk oxygen nanobubbles.

ABSTRACT: Bulk nanobubbles, measuring less than 200 nm in water, have shown their salient properties in promoting growth in various species of plants and orthodox seeds, and as potential drug-delivery carriers in medicine. Studies of recalcitrant seeds have reported markedly increased germination rates with gibberellin treatment; however, neither the mechanism promoting germination nor the implication for food safety is well elucidated. In our study, recalcitrant wasabi (Eutrema japonicum) seeds treated with bulk oxygen nanobubbles (BONB) containing K⁺, Na⁺, and Cl^- (BONB-KNaCl) showed significantly accelerated germination. As germination progressed, 99% of K⁺ ions in the BONB-KNaCl medium were absorbed by the seeds, whereas Ca²⁺ ions were released. These results suggest that the germination mechanism involves the action of K⁺ channels for migration of K⁺ ions down their concentration gradient and Ca²⁺ pumps for the movement of Ca²⁺ ions, the first potential discovery in germination promotion in recalcitrant seeds using nutrient solutions with BONB-KNaCl.

SUBMITTER: Kim M 

PROVIDER: S-EPMC9971192 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature