Phenotypic characterization of single CD4+ T cells harboring genetically intact and inducible HIV genomes.

ABSTRACT: The phenotype of the rare HIV-infected cells persisting during antiretroviral therapies (ART) remains elusive. We developed a single-cell approach that combines the phenotypic analysis of HIV-infected cells with near full-length sequencing of their associated proviruses to characterize the viral reservoir in 6 male individuals on suppressive ART. We show that individual cells carrying clonally expanded identical proviruses display very diverse phenotypes, indicating that cellular proliferation contributes to the phenotypic diversification of the HIV reservoir. Unlike most viral genomes persisting on ART, inducible and translation-competent proviruses rarely present large deletions but are enriched in defects in the Ψ locus. Interestingly, the few cells harboring genetically intact and indu

SUBMITTER: Dufour C 

PROVIDER: S-EPMC9971253 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature