Development of a novel ex vivo organ culture system to improve preservation methods of regenerative tissues.

ABSTRACT: Recent advances in regenerative technology have made the regeneration of various organs using pluripotent stem cells possible. However, a simpler screening method for evaluating regenerated organs is required to apply this technology to clinical regenerative medicine in the future. We have developed a simple evaluation method using a mouse tooth germ culture model of organs formed by epithelial-mesenchymal interactions. In this study, we successfully established a simple method that controls tissue development in a temperature-dependent manner using a mouse tooth germ ex vivo culture model. We observed that the development of the cultured tooth germ could be delayed by low-temperature culture and resumed by the subsequent culture at 37 °C. Furthermore, the optimal temperature for the long-

SUBMITTER: Yuta T 

PROVIDER: S-EPMC9971270 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature