Single-cell RNA-seq analysis reveals dual sensing of HIV-1 in blood Axl⁺ dendritic cells.

ABSTRACT: Sensing of incoming viruses is a pivotal task of dendritic cells (DCs). Human primary blood DCs encompass various subsets that are diverse in their susceptibility and response to HIV-1. The recent identification of the blood Axl⁺DC subset, endowed with unique capacities to bind, replicate, and transmit HIV-1 prompted us to evaluate its anti-viral response. We demonstrate that HIV-1 induced two main broad and intense transcriptional programs in different Axl⁺DCs potentially induced by different sensors; an NF-κB-mediated program that led to DC maturation and efficient CD4⁺ T cell activation, and a program mediated by STAT1/2 that activated type I IFN and ISG responses. These responses were absent from cDC2 exposed to HIV-1 except when viral replication was a

SUBMITTER: Brouiller F 

PROVIDER: S-EPMC9971904 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature