Identification of hub genes regulating isoflavone accumulation in soybean seeds via GWAS and WGCNA approaches.

ABSTRACT:

Introduction

Isoflavones are the secondary metabolites synthesized by the phenylpropanoid biosynthesis pathway in soybean that benefits human and plant health.

Methods

In this study, we have profiled seed isoflavone content by HPLC in 1551 soybean accessions grown in Beijing and Hainan for two consecutive years (2017 and 2018) and in Anhui for one year (2017).

Results

A broad range of phenotypic variations was observed for individual and total isoflavone (TIF) content. The TIF content ranged from 677.25 to 5823.29 µg g^-1 in the soybean natural population. Using a genome-wide association study (GWAS) based on 6,149,599 single nucleotide polymorphisms (SNPs), we identified 11,704 SNPs significantly associated with isoflavone contents; 75% of them were located

SUBMITTER: Azam M 

PROVIDER: S-EPMC9971994 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature