Identification and Functional Characterization of Fungal Chalcone Synthase and Chalcone Isomerase.

ABSTRACT: By mining fungal genomic information, a noncanonical iterative type I PKS fused with an N-terminal adenylation-thiolation didomain, which catalyzes the formation of naringenin chalcone, was found. Structural prediction and molecular docking analysis indicated that a C-terminal thioesterase domain was involved in the Claisen-type cyclization. An enzyme responsible for formation of (2S)-flavanone in the biosynthesis of fungal flavonoids was also identified. Collectively, these findings demonstrate unprecedented fungal biosynthetic machinery leading to plant-like metabolites.

SUBMITTER: Furumura S 

PROVIDER: S-EPMC9972472 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature