A new intronic quantitative PCR method led to the discovery of transformation from human ascites to murine malignancy in a mouse model.

ABSTRACT:

Purpose

To establish a fast and accurate detection method for interspecies contaminations in the patient-derived xenograft (PDX) models and cell lines, and to elucidate possible mechanisms if interspecies oncogenic transformation is detected.

Methods

A fast and highly sensitive intronic qPCR method detecting Gapdh intronic genomic copies was developed to quantify if cells were human or murine or a mixture. By this method, we documented that murine stromal cells were abundant in the PDXs; we also authenticated our cell lines to be human or murine.

Results

In one mouse model, GA0825-PDX transformed murine stromal cells into a malignant tumorigenic murine P0825 cell line. We traced the timeline of this transformation and discovered three subpopulations descended from the

SUBMITTER: Jin J 

PROVIDER: S-EPMC9972586 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature