VLPs containing stalk domain and ectodomain of matrix protein 2 of influenza induce protection in mice.

ABSTRACT:

Background

As a result of antigenic drift, current influenza vaccines provide limited protection against circulating influenza viruses, and vaccines with broad cross protection are urgently needed. Hemagglutinin stalk domain and ectodomain of matrix protein 2 are highly conserved among influenza viruses and have great potential for use as a universal vaccine.

Methods

In this study, we co-expressed the stalk domain and M2e on the surface of cell membranes and generated chimeric and standard virus-like particles of influenza to improve antigen immunogenicity. We subsequently immunized BALB/c mice through intranasal and intramuscular routes.

Results

Data obtained demonstrated that vaccination with VLPs elicited high levels of serum-specific IgG (approximately 30-fold hig

SUBMITTER: Shi L 

PROVIDER: S-EPMC9972598 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature