Synovial fibroblasts assume distinct functional identities and secrete R-spondin 2 in osteoarthritis.

ABSTRACT:

Objectives

Synovium is acutely affected following joint trauma and contributes to post-traumatic osteoarthritis (PTOA) progression. Little is known about discrete cell types and molecular mechanisms in PTOA synovium. We aimed to describe synovial cell populations and their dynamics in PTOA, with a focus on fibroblasts. We also sought to define mechanisms of synovial Wnt/β-catenin signalling, given its emerging importance in arthritis.

Methods

We subjected mice to non-invasive anterior cruciate ligament rupture as a model of human joint injury. We performed single-cell RNA-sequencing to assess synovial cell populations, subjected Wnt-GFP reporter mice to joint injury to study Wnt-active cells, and performed intra-articular injections of the Wnt agonist R-spondin 2 (Rspo2) to

SUBMITTER: Knights AJ 

PROVIDER: S-EPMC9972892 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature