Characterization of Escherichia coli and Other Enterobacterales Resistant to Extended-Spectrum Cephalosporins Isolated from Dairy Manure in Ontario, Canada.

ABSTRACT: Extended-spectrum cephalosporins (ESCs) resistance genes, such as bla_CTX-M, bla_CMY, and bla_SHV, have been found regularly in bacteria from livestock. However, information on their distribution in dairy cattle in Canada and on the associated genome sequences of ESC-resistant Enterobacterales is sparse. In this study, the diversity and distribution of ESC-resistant Escherichia coli throughout manure treatments in six farms in Southern Ontario were assessed over a one-year period, and their ESC-resistance plasmids were characterized. The manure samples were enriched using selective media. The resulting isolates were screened via polymerase chain reaction for bla_CTX-M, bla_CMY, and bla_SHV

SUBMITTER: Anderson REV 

PROVIDER: S-EPMC9972979 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature