Impact of Growth Rate on the Protein-mRNA Ratio in Pseudomonas aeruginosa.

ABSTRACT: Our understanding of how bacterial pathogens colonize and persist during human infection has been hampered by the limited characterization of bacterial physiology during infection and a research bias toward in vitro, fast-growing bacteria. Recent research has begun to address these gaps in knowledge by directly quantifying bacterial mRNA levels during human infection, with the goal of assessing microbial community function at the infection site. However, mRNA levels are not always predictive of protein levels, which are the primary functional units of a cell. Here, we used carefully controlled chemostat experiments to examine the relationship between mRNA and protein levels across four growth rates in the bacterial pathogen Pseudomonas aeruginosa. We found a genome-wide positive cor

SUBMITTER: Zhang M 

PROVIDER: S-EPMC9973009 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature