Developing a Temperature-Inducible Transcriptional Rheostat in Neurospora crassa.

ABSTRACT: Heat shock protein (HSP)-encoding genes (hsp), part of the highly conserved heat shock response (HSR), are known to be induced by thermal stress in several organisms. In Neurospora crassa, three hsp genes, hsp30, hsp70, and hsp80, have been characterized; however, the role of defined cis elements in their responses to discrete changes in temperature remains largely unexplored. To fill this gap, while also aiming to obtain a reliable fungal heat shock-inducible system, we analyzed different sections of each hsp promoter by assessing the expression of real-time transcriptional reporters. Whereas all three promoters and their resected versions were acutely induced by high temperatures, only hsp30 displayed a broad range of expression and

SUBMITTER: Tabilo-Agurto C 

PROVIDER: S-EPMC9973361 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature