Identification of an ATP-driven, osmoregulated glycine betaine transport system in Listeria monocytogenes.

ABSTRACT: The ability of the gram-positive, food-borne pathogen Listeria monocytogenes to tolerate environments of elevated osmolarity and reduced temperature is due in part to the transport and accumulation of the osmolyte glycine betaine. Previously we showed that glycine betaine transport was the result of Na(+)-glycine betaine symport. In this report, we identify a second glycine betaine transporter from L. monocytogenes which is osmotically activated but does not require a high concentration of Na(+) for activity. By using a pool of Tn917-LTV3 mutants, a salt- and chill-sensitive mutant which was also found to be impaired in its ability to transport glycine betaine was isolated. DNA sequence analysis of the region flanking the site of transposon insertion revealed three open reading frames homo

SUBMITTER: Ko R 

PROVIDER: S-EPMC99739 | biostudies-literature | 1999 Sep

REPOSITORIES: biostudies-literature