Evolutional transition of HBV genome during the persistent infection determined by single-molecule real-time sequencing.

ABSTRACT:

Background

Although HBV infection is a serious health issue worldwide, the landscape of HBV genome dynamics in the host has not yet been clarified. This study aimed to determine the continuous genome sequence of each HBV clone using a single-molecule real-time sequencing platform, and clarify the dynamics of structural abnormalities during persistent HBV infection without antiviral therapy.

Patients and methods

Twenty-five serum specimens were collected from 10 untreated HBV-infected patients. Continuous whole-genome sequencing of each clone was performed using a PacBio Sequel sequencer; the relationship between genomic variations and clinical information was analyzed. The diversity and phylogeny of the viral clones with structural variations were also analyzed.

Results</

SUBMITTER: Arasawa S 

PROVIDER: S-EPMC9974078 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature