Quality analysis of genomic DNA and authentication of fisheries products based on distinct methods of DNA extraction.

ABSTRACT: Molecular genetic techniques are an effective monitoring tool, but high-quality DNA samples are usually required. In this study, we compared three different protocols of DNA extraction: NaCl (saline); phenol-chloroform and commercial kit (Promega)-from three biological tissues of five individuals of Lutjanus purpureus under two methods of storage. The evaluated items included DNA concentration and purity, processing time and cost, as well as the obtaining of functional sequences. The highest average values of DNA concentration were obtained using the saline procedure and the commercial kit. Pure DNA was only obtained using the saline protocol, evaluated by the ratio of 260/280. The saline and phenol-chloroform protocols were the least expensive methods. The commercial kit costs are counter

SUBMITTER: Lutz I 

PROVIDER: S-EPMC9974130 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature