A proteomic analysis of NETosis in trauma: Emergence of serpinB1 as a key player.

ABSTRACT:

Background

Release of neutrophil extracellular traps (NETosis) may mediate postinjury organ dysfunction, but mechanisms remain unclear. The intracellular serine protease inhibitor (serpin) B1 is vital to neutrophil function and has been shown to restrict NETosis in inflammatory settings. In this study, we used discovery proteomics to identify the proteomic signature of trauma-induced NETosis. We hypothesized that serpinB1 would be a major component of this NET protein profile and associated with adverse outcomes.

Methods

This was a post hoc analysis of data collected as part of the COMBAT randomized clinical trial. Blood was collected from injured patients at a single Level I Trauma Center. Proteomic analyses were performed through targeted liquid chromatography coupled with

SUBMITTER: Schaid TR 

PROVIDER: S-EPMC9974543 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature