Blood transcriptome responses to PFOA and GenX treatment in the marsupial biomedical model Monodelphis domestica.

ABSTRACT: Introduction: Perfluoroalkyl and poly-fluoroalkyl substances (PFASs) are widely used in industrial and consumer products. Due to their environmental persistence and bioaccumulation, PFASs can be found in the blood of humans and wild animals all over the world. Various fluorinated alternatives such as GenX have been developed to replace the long-chain PFASs, but there is limited information about their potential toxicity. Methods:The current study developed blood culture protocols to assess the response to toxic compounds in the marsupial, Monodelphis domestica. After whole-blood culture conditions were tested and optimized, changes in gene expression in response to PFOA and GenX treatment were assessed. Results: More than 10,000 genes were expressed in the blood

SUBMITTER: Cao W 

PROVIDER: S-EPMC9974665 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature