Soluble peptidoglycan fragments produced by <i>Limosilactobacillus fermentum</i> with antiproliferative activity are suitable for potential therapeutic development: A preliminary report.

ABSTRACT: Currently, the use of probiotic strains and their products represents a promising innovative approach as an antagonist treatment against many human diseases. Previous studies showed that a strain of Limosilactobacillus fermentum (LAC92), previously defined as Lactobacillus fermentum, exhibited a suitable amensalistic property. The present study aimed to purify the active components from LAC92 to evaluate the biological properties of soluble peptidoglycan fragments (SPFs). The cell-free supernatant (CFS) and bacterial cells were separated after 48 h of growth in MRS medium broth and treated for isolation of SPFs. Antimicrobial activity and proliferation analysis on the human cell line HTC116 were performed using technologies such as xCELLigence, count and viability, and clonog

SUBMITTER: Fuochi V 

PROVIDER: S-EPMC9975264 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature