Chemical modification by peroxynitrite enhances TLR4 activation of the grass pollen allergen Phl p 5.

ABSTRACT: The chemical modification of aeroallergens by reactive oxygen and nitrogen species (ROS/RNS) may contribute to the growing prevalence of respiratory allergies in industrialized countries. Post-translational modifications can alter the immunological properties of proteins, but the underlying mechanisms and effects are not well understood. In this study, we investigate the Toll-like receptor 4 (TLR4) activation of the major birch and grass pollen allergens Bet v 1 and Phl p 5, and how the physiological oxidant peroxynitrite (ONOO^-) changes the TLR4 activation through protein nitration and the formation of protein dimers and higher oligomers. Of the two allergens, Bet v 1 exhibited no TLR4 activation, but we found TLR4 activation of Phl p 5, which increased after modification with

SUBMITTER: Reinmuth-Selzle K 

PROVIDER: S-EPMC9975604 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature