L-kynurenine induces NK cell loss in gastric cancer microenvironment via promoting ferroptosis.

ABSTRACT:

Background

Natural killer (NK) cells play a major role in body's fighting against various types of cancers. Their infiltration in the tumor microenvironment (TME) of gastric cancer (GC) are significantly decreased, which has been reported as a robust prognostic marker. However, the causes leading to NK cells loss in GC TME remains poorly understood.

Methods

We constructed a non-contact co-culturing system and humanized xenograft tumor mice model to detect the influence of GC microenvironment on NK-92 or primary human NK cells viability by flow cytometry. Then through using the specific inhibitors for different types of cell death and examining the surrogate markers, we confirmed ferroptosis in NK cells. Inspired by the accidental discoveries, we constructed a NK-92 cell stra

SUBMITTER: Cui JX 

PROVIDER: S-EPMC9976385 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature