Aryl hydrocarbon receptor nuclear translocator limits the recruitment and function of regulatory neutrophils against colorectal cancer by regulating the gut microbiota.

ABSTRACT:

Background

Although the role and mechanism of neutrophils in tumors have been widely studied, the precise effects of aryl hydrocarbon receptor nuclear translocator (ARNT) on neutrophils remain unclear. In this study, we investigated the roles of ARNT in the function of CD11b⁺Gr1⁺ neutrophils in colitis-associated colorectal cancer.

Methods

Wild-type (WT), ARNT myeloid-specific deficient mice and a colitis-associated colorectal cancer mouse model were used in this study. The level and functions of CD11b⁺Gr1⁺ cells were evaluated by flow cytometry and confocal microscopy.

Results

We found that ARNT deficiency drives neutrophils recruitment, neutrophil extracellular trap (NET) development, inflammatory cytokine secretion and su

SUBMITTER: Bi Y 

PROVIDER: S-EPMC9976387 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature