Screening of core genes prognostic for sepsis and construction of a ceRNA regulatory network.

ABSTRACT:

Objective

To screen out core genes potentially prognostic for sepsis and construct a competing endogenous RNA (ceRNA) regulatory network.

Methods

Subjects included in this project were 23 sepsis patients and 10 healthy people. RNA-seq for lncRNA, miRNA and mRNA was performed in the peripheral blood samples. Differentially expressed RNAs (DER) were screened out for further analysis. GO annotation and GSEA functional clustering were performed to view the functional enrichment of DEmRNAs. Core genes of prognostic significance were screened out with the weighted correlation network analysis (WGCNA). Meta-analysis and Survival analysis was devised in different microarray datasets. RT-qPCR was conducted to validate these core genes. A ceRNA network was accordingly constructed acco

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC9976425 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature