Cost-effective duplex Kompetitive Allele Specific PCR markers for homologous genes facilitating wheat breeding.

ABSTRACT:

Background

Owing to successful cloning of wheat functional genes in recent years, more traits can be selected by diagnostic markers, and consequently, effective molecular markers will be powerful tools in wheat breeding programs.

Results

The present study proposed a cost-effective duplex Kompetitive Allele Specific PCR (dKASP) marker system that combined multiplex PCR and KASP™ technology to yield twice the efficiency at half the cost compared with the common KASP™ markers and provide great assistance in breeding selection. Three dKASP markers for the major genes controlling plant height (Rht-B1/Rht-D1), grain hardness (Pina-D1/Pinb-D1), and high-molecular-weight glutenin subunits (Glu-A1/Glu-D1) were successfully developed and applied in approved wheat varieties growing in

SUBMITTER: Jiang P 

PROVIDER: S-EPMC9976436 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature