Exosome-mediated crosstalk between epithelial cells amplifies the cell injury cascade in CaOx stone formation.

ABSTRACT:

Background

Calcium oxalate (CaOx) stone disease is found worldwide. To explore the role of exosomes as a mediator of intercellular crosstalk during CaOx stone formation, we conducted this study, which may provide a new insight into the treatment and prevention of CaOx stones.

Methods

Exosomes derived from HK2 cells with (EXO(S)) or without (EXO(C))CaOx crystal stimulation were cocultured with normal tubular epithelial cells and subcapsularly injected into rat kidneys. Then, oxidative stress levels, the MAPK signalling pathway and osteogenic changes were detected via qPCR, Western blotting, immunofluorescence and immunohistochemical staining. In vivo fluorescence imaging and exosome internalization assays showed the absorption and utilization of exosomes.

Results

EXO(S

SUBMITTER: Yang Y 

PROVIDER: S-EPMC9976448 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature