Automating iPSC generation to enable autologous photoreceptor cell replacement therapy.

ABSTRACT:

Background

Inherited retinal degeneration is a leading cause of incurable vision loss in the developed world. While autologous iPSC mediated photoreceptor cell replacement is theoretically possible, the lack of commercially available technologies designed to enable high throughput parallel production of patient specific therapeutics has hindered clinical translation.

Methods

In this study, we describe the use of the Cell X precision robotic cell culture platform to enable parallel production of clinical grade patient specific iPSCs. The Cell X is housed within an ISO Class 5 cGMP compliant closed aseptic isolator (Biospherix XVivo X2), where all procedures from fibroblast culture to iPSC generation, clonal expansion and retinal differentiation were performed.

Results

SUBMITTER: Bohrer LR 

PROVIDER: S-EPMC9976478 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature