Assembly and operation of an imaging system for long-term monitoring of bioluminescent and fluorescent reporters in plants.

ABSTRACT:

Background

Non-invasive reporter systems are powerful tools to query physiological and transcriptional responses in organisms. For example, fluorescent and bioluminescent reporters have revolutionized cellular and organismal assays and have been used to study plant responses to abiotic and biotic stressors. Integrated, cooled charge-coupled device (CCD) camera systems have been developed to image bioluminescent and fluorescent signals in a variety of organisms; however, these integrated long-term imaging systems are expensive.

Results

We have developed self-assembled systems for both growing and monitoring plant fluorescence and bioluminescence for long-term experiments under controlled environmental conditions. This system combines environmental growth chambers with high-se

SUBMITTER: Sorkin ML 

PROVIDER: S-EPMC9976486 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature