Serum-integrated omics reveal the host response landscape for severe pediatric community-acquired pneumonia.

ABSTRACT:

Objective

Community-acquired pneumonia (CAP) is the primary cause of death for children under five years of age globally. Hence, it is essential to investigate new early biomarkers and potential mechanisms involved in disease severity.

Methods

Proteomics combined with metabolomics was performed to identify biomarkers suitable for early diagnosis of severe CAP. In the training cohort, proteomics and metabolomics were performed on serum samples obtained from 20 severe CAPs (S-CAPs), 15 non-severe CAPs (NS-CAPs) and 15 healthy controls (CONs). In the verification cohort, selected biomarkers and their combinations were validated using ELISA and metabolomics in an independent cohort of 129 subjects. Finally, a combined proteomics and metabolomics analysis was performed to underst

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9976684 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature