Multiomics approach to profiling Sertoli cell maturation during development of the spermatogonial stem cell niche.

ABSTRACT: Spermatogonial stem cells (SSCs) are the basis of spermatogenesis, a complex process supported by a specialized microenvironment, called the SSC niche. Postnatal development of SSCs is characterized by distinct metabolic transitions from prepubertal to adult stages. An understanding of the niche factors that regulate these maturational events is critical for the clinical application of SSCs in fertility preservation. To investigate the niche maturation events that take place during SSC maturation, we combined different '-omics' technologies. Serial single cell RNA sequencing analysis revealed changes in the transcriptomes indicative of niche maturation that was initiated at 11 years of age in humans and at 8 weeks of age in pigs, as evident by Monocle analysis of Sertoli cells and peritubu

SUBMITTER: Voigt AL 

PROVIDER: S-EPMC9976880 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature