Passer, a highly active transposon from a fish genome, as a potential new robust genetic manipulation tool.

ABSTRACT: The discovery of new, active DNA transposons can expand the range of genetic tools and provide more options for genomic manipulation. In this study, a bioinformatics analysis suggested that Passer (PS) transposons, which are members of the pogo superfamily, show signs of recent and current activity in animals and may be active in some species. Cell-based transposition assays revealed that the native PS transposases from Gasterosteus aculeatus and Danio rerio displayed very high activity in human cells relative to the Sleeping Beauty transposon. A typical overproduction inhibition phenomenon was observed for PS, and transposition capacity was decreased by ∼12% with each kilobase increase in the insertion size. Furthermore, PS exhibited a pronounced integration preference for genes and their

SUBMITTER: Wang S 

PROVIDER: S-EPMC9976928 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature