Laser Microdissection for Species-Agnostic Single-Tissue Applications.

ABSTRACT: Single-cell methodologies have revolutionized the analysis of the transcriptomes of specific cell types. However, they often require species-specific genetic "toolkits," such as promoters driving tissue-specific expression of fluorescent proteins. Further, protocols that disrupt tissues to isolate individual cells remove cells from their native environment (e.g., signaling from neighbors) and may result in stress responses or other differences from native gene expression states. In the present protocol, laser microdissection (LMD) is optimized to isolate individual nematode tail tips for the study of gene expression during male tail tip morphogenesis. LMD allows the isolation of a portion of the animal without the need for cellular disruption or species-specific toolkits and is thus applic

SUBMITTER: Woronik A 

PROVIDER: S-EPMC9976942 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature